【Abstract】 Objective To study the effects of Boettcherisca peregrine pupae frozen storage on the reproduction of Nasonia vitripennis. Methods The developmental duration, body length, female longevity and reproduction of the parasitoid were tested after the pupae of   B.peregrine  were  frozen  for different times at -20 ℃ and -70 ℃.  Results The developmental durations of N.vitripennis progeny (F1) at B.peregrine pupae stored at -20 ℃ and -70 ℃ were 12-13 d, the body length of female and male progeny were about 2 mm and 1.4 mm, respectively, and the longevity of female progeny was about 11 days. The above indexes had no significant difference with the control. The parasitic ratio of F1 N.vitripennis to natural B.peregrine pupae was 90%-97%, and the emergence ratio of F1 progeny was about 80%-87%. There were about 40 progeny parasitoids emerged from one fly pupa, and the female ratio was about 90%, the examined indexes had no significant difference with the control. Conclusion Frozen storage of B.peregrine pupae had no effect on the development and quality of N.vitripennis progeny.

Objective The parasitic behavior of female Nasonia vitripennis was studied.Methods Dissecting the parasitized fly pupae,the eggs number of female N.vitripennis laid in different parts of the three species of fly pupae was counted.The effect of parasitic times on the fecundity of female N.vitripennis was studied.Results Feeding with 20% honey water,the longevity of female was prolonged significantly.The female N.vitripennis mainly laid eggs in the abdomen of its host flies,especially on the sternites.Mating or not had no significant effect on the parasitic ability of the female parasitoid,but the fecundity of the parasitoid was decreased with the increase of the parasitic times.Conclusion The parasitic ability of female N.vitripennis had correlation with the parasitic times.